FAQ dNTPs Bioline

Text nur auf Englisch verfügbar.

  1. Introduction
  2. Performance and Sensitivity
  3. Purity
  4. Quality Control
  5. Configuration
  6. Extended Storage Life
  7. Bulk Orders
  8. Table of Physical Constants, Spectral & HPLC Analysis
  9. dNTP Mix
  10. dNTP Set
  11. dUTP Mix & ACGU + T Mix
  12. Ordering Information
  13. Related Products

     

1. Introduction

The importance of dNTP quality in today’s molecular biology is often overlooked. Bioline’s ultra pure dNTPs are enzymatically manufactured to maximise purity and quality. Our dNTPs will work even under the most difficult and sensitive situations, such as long-distance & qPCR, Reverse Transcription and DHPLC.

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2. Performance and Sensitivity

Bioline dNTPs meet exacting standards and are suitable for the most demanding applications such as long distance PCR and DNA synthesis, Real-Time PCR and Reverse Transcription. Bioline dNTPs can be employed in highly sensitive assays such as low-copy or rare-message assays.

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3. Purity

Bioline dNTPs are enzymatically synthesised from dNMPs by enzymatic phosphorylation. This process eliminates impurities and PCR inhibitors such as modified nucleotides, inorganic pyrophosphates and tetraphosphates. The dNTPs are purified with preparative HPLC and possess at least 99% purity.

DNA fragments amplified using Bioline dNTPs have been assayed for use on DHPLC. dNTP purity is a critical consideration for DHPLC usage, as there must be no contamination by monophosphates or incorrectly synthesised/ modified bases. Contaminating nucleotides or derivatives may cause premature strand termination and result in unwanted peaks.

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4. Quality Control

dNTPs are tested for the absence of DNase, RNase, Protease and nicking activity. Tests to confirm the absence of human and bacterial DNA are also carried out. Each batch is validated rigorously for purity by HPLC, and tested for performance in a wide range of PCR templates, including a long-distance 20kb functional test.

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5. Configuration

Bioline dNTPs are available as convenient 100 mM sets or as 10, 40 and 100 mM ready-to-go mixes, which are designed to save time and reduce the risk of contamination. The dNTP solutions are ready-to-use at pH 7.0 in Lithium salts.

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6. Extended Storage Life

Bioline dNTPs have the distinct advantage of being presented as Lithium Salts as opposed to Sodium Salts. Lithium presentation makes dNTPs more resistant to repeated freeze/ thawing. A Lithium salt solution is also an unsuitable environment for bacteria and fungi, so the dNTPs remain sterile throughout the storage and usage period. Bioline dNTPs are stable for 12 months when stored in a -20ºC constant-temperature freezer.

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7. Bulk Orders

Bioline can accommodate multi-litre orders for dNTPs, so we welcome bulk enquiries.

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8. Table of Physical Constants, Spectral & HPLC Analysis

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9. dNTP Mix

  • Ultra-pure: > 99% triphosphate by HPLC.
  • Enhanced storage life.
  • Convenient solutions at pH 7.0.

A ready-to-use molecular-grade solution containing dA, dC, dG and dT premixed and ready for use in DNA polymerisation reactions, DNA-labelling and sequencing processes. The mix is designed to save hands-on time for researchers and minimises the possibility of contamination.

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10. dNTP Set

  • Ultra-pure: > 99% triphosphate by HPLC.
  • Enhanced storage life.
  • Convenient solutions at pH 7.0.

Ready-to-use molecular-grade dNTP solutions supplied as sets: dA, dC, dG and dT, for use in DNA polymerisation reactions, DNAlabelling and sequencing processes. Dependable PCR grade.

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11. dUTP Mix & ACGU + T Mix

  • Used in conjunction with UNG to prevent carry-over of amplified products into subsequent amplifications.
  • Convenient pre-optimised, premixed PCR grade solutions containing dATP, dCTP, dGTP and dUTP, as well as dTTP in the case of ACGU + T Mix.
  • Applications: Real-Time PCR.

Bioline dUTP Mix and ACGU + T Mix enable users to employ the efficient strategy of incorporating dUTP into amplification reactions. In this process, dUTP replaces dTTP in amplified products. Before a subsequent DNA amplification is commenced, treatment with Uracil DNA Glycosylase (UNG) will result in the selective cleavage of the Uracil base in the dUTP-containing DNA, therefore preventing carry-over contaminations.

Note: dUTP Mix is the standard preparation recommended. However, please use ACGU + T Mix for >500 bp amplifications.
* Related product: Uracil DNA Glycosylase (UNG), BIO-27044

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12. Ordering Information

Product  Pack Size  Final Conc.  Presentation  Catalog No.
dNTP Set4 x 25 µmo100 mM4 x 250 µlBIO-39025
dNTP Set4 x 100 µmol100 mM4 x 4 x 250 µlBIO-39026
dNTP Set4 x 500 µmol100 mM4 x 20 x 250 µl   BIO-39027
dNTP Mix10 µmol10 mM total1 mlBIO-39044
dNTP Mix10 x 10 µmol   10 mM total10 x 1000 µlBIO-39053
dNTP Mix20 x 10 µmol10 mM total20 x 1000 µlBIO-39054
dNTP Mix20 µmol40 mM total500 µlBIO-39043
dNTP Mix50 µmol100 mM total    500 µlBIO-39028
dNTP Mix200 µmol100 mM total4 x 500 µlBIO-39029
dATP, Individual  25 µmol100 mM250 µlBIO-39036
dCTP, Individual25 µmol100 mM250 µlBIO-39038
dGTP, Individual25 µmol100 mM250 µlBIO-39037
dTTP, Individual25 µmol100 mM250 µlBIO-39039
dUTP, Individual25 µmol100 mM250 µlBIO-39035
dITP, Individual25 µmol100 mM250 µlBIO-39032
dUTP Mix (50 x Sol.)
(10 mM each dA, dC,
dG, 20 mM dUTP) 
25 µmol50 mM total500 µlBIO-39041
ACGU & T mix (50 x Sol.)  
(10 mM each dA, dC,
dG, 7 mM dt., 3 MM dU)
20 µmol40 mM total500 µlBIO-39041

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13. Related Products

Product  Pack Size  Final Conc.  Presentation  Catalog No.
Uracil DNA Glycosylase (UNG)  500 Units1 u/µl500 µlBIO-27044
NTP Set (ATP, CTP, GTP, UTP)  4 x 25 µmol   100 mM4 x 250 µlBIO-39052
NTP Mix100 µmol100 mM total    1 mlBIO-39050

* PCR is a registered Trademark Roche Molecular Systems.

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Kontakt

Sie erreichen uns telefonisch unter der Rufnummer
+49 (0) 83 82 / 5 04 36 58
oder per E-Mail an 
info(at)amplifa.com.

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Letzte Aktualisierung: 05.09.2012